ABSTRACT
PURPOSE: To evaluate two treatment modalities of dry socket in rats and to discuss the first findings of the molecular analysis in this experimental model. METHODS: 84 rats underwent a tooth extraction were divided in 4 groups: I-uninfected socket (control), II-infected socket without any treatment, III-infected socket treated with irrigation of 2 percent sodium iodide and 3 percent hydrogen peroxide solution, IV-infected socket submitted to curettage, irrigation with physiological saline solution and fulfilled with metronidazole paste as base. The groups were subdivided in postoperative sacrifice periods: 6/15/28 days. A quantitative and a qualitative microscopic analysis was performed. Also, a quantitative analysis was performed using a RealTimePCR to evaluate the genes expression in the wound healing: Collagen Type I/COL-I, vascular endothelial growth factor/VEGF, osteocalcin/OCN, alkaline phosphatase/ALP, runt-related transcription factor 2/RUNX2 and tumor necrosis factor alpha/TNF-α. RESULTS: The group I showed higher bone formation, followed by groups IV, III, II respectively. The group II presented higher inflammatory infiltrate and the wound healing was delayed compared with other groups. It was obtained a significant positive correlation between bone neoformation and the expression of OCN and RUNX2, inflammatory infiltrate with TNF-α and a negative correlation between bone neoformation and TNF-α. CONCLUSION: No significant difference was found between the treatments.
OBJETIVO: Avaliar duas modalidades de tratamento da alveolite em ratos e discutir os primeiros achados de uma análise molecular neste modelo experimental. MÉTODOS: 84 ratos foram submetidos a uma extração dentária e foram divididos em quatro grupos: I- alvéolo não infectado (controle), II- alvéolo infectado sem tratamento, III- alvéolo infectado tratado com irrigação de iodeto de sódio a 2 por cento e solução de peróxido de hidrogênio a 3 por cento, IV- alvéolo infectado submetido à curetagem, irrigação com solução salina fisiológica e preenchimento com pasta a base de metronidazol. Os grupos foram subdivididos em períodos de sacrifício pós-operatório: 6/15/28 dias. Uma análise quantitativa e qualitativa microscópica foi realizada. Além disso, uma análise quantitativa foi realizada utilizando RealTimePCR para avaliar a expressão de genes no reparo alveolar: o colágeno tipo I / COL-I, o fator de crescimento endotelial vascular / VEGF, osteocalcina / OCN, fosfatase alcalina / ALP, fator de transcrição runt relacionados 2 / RUNX2 e fator de necrose tumoral alfa / TNF-α. RESULTADOS: O grupo I mostrou maior formação óssea, seguido pelos grupos IV, III, II, respectivamente. O grupo II apresentou maior infiltrado inflamatório e a cicatrização foi atrasada em comparação com outros grupos. Foi obtida uma correlação positiva entre a neoformação óssea e a expressão de OCN e RUNX2, infiltrado inflamatório com TNF-α e uma correlação negativa entre a neoformação óssea e TNF-α. CONCLUSÃO: Nenhuma diferença significativa foi encontrada entre os tratamentos.
Subject(s)
Animals , Male , Rats , Anti-Infective Agents/therapeutic use , Dry Socket/drug therapy , Osteogenesis/drug effects , Wound Healing/drug effects , Bone Density , Dry Socket/pathology , Hydrogen Peroxide/therapeutic use , Metronidazole/therapeutic use , Rats, Wistar , Real-Time Polymerase Chain Reaction , Sodium Iodide/therapeutic use , Tumor Necrosis Factor-alpha/metabolism , Vascular Endothelial Growth Factor A/metabolismABSTRACT
The pathogenesis of alveolitis is not well known and therefore experimental situations that mimic some features of this disease should be developed. OBJECTIVE: In this study, the evolution of the experimentally induced infection in rat sockets is characterized, which leads to clinical signs of suppurative alveolitis with remarkable wound healing disturbs. MATERIAL AND METHODS: Non-infected (Group I) and experimentally infected sockets in Rattus novergicus (Group II) were histometrically evaluated regarding the kinetics of alveolar healing. In addition, the characterization of the present bacteria in inoculation material and the serum levels of C-reactive protein (CRP) were performed. The detected species were Capnocytophaga ochracea, Fusobacterium nucleatum ss nucleatum, Prevotella melaninogenica, Streptococcus anginosus, Treponema socranskii and Streptococcus sanguis. RESULTS: All experimentally infected rats developed suppurative alveolitis, showing higher levels of CRP in comparison to those non-infected ones. Furthermore, infected rats presented a significant delayed wound healing as measured by the histometric analysis (higher persistent polymorphonuclear infiltrate and lower density of newly formed bone). CONCLUSION: These findings indicate that rat sockets with experimentally induced infection produced higher levels of serum CRP, showing the potential of disseminated infection and a disturb in the alveolar repair process in an interesting experimental model for alveolitis studies.
Subject(s)
Animals , Male , Rats , Dry Socket/pathology , Tooth Extraction/adverse effects , Tooth Socket/pathology , Wound Healing , Bacterial Infections/pathology , C-Reactive Protein/analysis , DNA Probes , Dry Socket/microbiology , Postoperative Period , Rats, Wistar , Time Factors , Tooth Socket/microbiologyABSTRACT
Neste estudo, foram analisados os resultados do pós-operatório de 511 exodontias, realizadas no ambulátorio de cirurgia da Faculdade de Odontologia da Pontifícia Universidade Católica do Rio Grande do Sul, no decorrer das disciplinas de Cirurgia I e II. Tais extraçöes foram efetuadas pelo alunos de 5§ e 6§ semestres do curso de Odontologia. Avaliou-se a incidência de alveolite de cada dentre, isoladamente, de cada grupo de dentes, por hemiarcadas, e no total. Os resultados sugerem que a menor experiência do profissional pode ser um dos fatores etiológicos da alveolite
Subject(s)
Humans , Male , Female , Surgery, Oral , Dry Socket/epidemiology , Dry Socket/etiology , Dry Socket/microbiology , Dry Socket/pathology , Dry Socket/therapyABSTRACT
Como hemos descripto, la alveolitis está en relación directa a los factores etiopatogénicos. Realizar un correcto diagnóstico y tratamiento aliviará al paciente de una complicación extremedamente dolorosa. Las medidas terapéuticas aconsejadas van dirigidas principalmente a acciones locales sobre las sistémicas